recombinant asprosin protein  (MedChemExpress)

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vitro overexpression of asprosin Asprosin levels measured using ELISA in HEK293T cell conditioned media collected 72 h post transfection with 10 μg of pcDNA3.1 (empty vector control) or pcDNA3.1-IL2sp-6his-Asprosin. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vitro, Over Expression, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: Viral overexpression of human asprosin results in increased appetite and body weight gain in lean mice (A and B) cumulative food intake and body weight change were measured 15- and 18-days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with Ad5-empty or Ad5-h FBN1 (3.6 × 10 9 pfu/mouse) viral vector. (C and D) Cumulative food intake and body weight change were measured 47 days and 60 days after 12-week-old, male, C57Bl/6 mice were tail-vein-injected with AAV8-empty or AAV8-IL2sp-6His-Asprosin (1 × 10 12 GC/mouse) viral vector. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: Over Expression, Injection, Plasmid Preparation

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vivo overexpression of Asprosin using adenoviral vector (Ad5) (A and B) Human asprosin levels detected in plasma of Ad5-empty (control) and Ad5-h FBN1 injected C57BL/6J male mice, 12 days (A) and 25 days (B) after viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in Ad5-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet: In vivo overexpression of Asprosin using adeno-associated viral vector 8 (A and B) Human asprosin levels detected in plasma of AAV8-empty (control) and AAV8-IL2sp-6His-Asprosin injected C57BL/6J male mice, 25 days (A) and 55 days (B) after adeno-associated viral vector transduction. Asprosin detection signal is plotted relative to the average background signal detected in AAV8-empty injected mice. Asterisk (∗) indicate the range of alpha; ∗ p<0.05, ∗∗ p<0.01, ∗∗∗ p<0.001, and ∗∗∗∗ p<0.0001, and (ns) denotes the statistical non significance as determined by student T-test. Data presented as mean ± SEM.

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: In Vivo, Over Expression, Plasmid Preparation, Injection, Transduction

Journal: STAR Protocols

Article Title: Overexpression and ELISA-based detection of asprosin in cultured cells and mice

doi: 10.1016/j.xpro.2022.101762

Figure Lengend Snippet:

Article Snippet: Recombinant human Asprosin , BioLegend , 761902.

Techniques: Concentration Assay, Recombinant, Protease Inhibitor, Transfection, Blocking Assay, Cell Culture, Plasmid Preparation